A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non‐invasive samples
Identifieur interne : 000232 ( Main/Exploration ); précédent : 000231; suivant : 000233A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non‐invasive samples
Auteurs : Tobias Bidon [Allemagne] ; Christiane Frosch [Allemagne] ; Hans G. Eiken [Norvège] ; Verena E. Kutschera [Allemagne] ; Snorre B. Hagen [Norvège] ; Siv G. Aarnes [Norvège] ; Steven R. Fain [États-Unis] ; Axel Janke [Allemagne] ; Frank Hailer [Allemagne]Source :
- Molecular Ecology Resources [ 1755-098X ] ; 2013-05.
Abstract
We report a new approach for molecular sex identification of extant Ursinae and Tremarctinae bears. Two Y‐specific fragments (SMCY and 318.2) and one X‐specific fragment (ZFX) are amplified in a multiplex PCR, yielding a double test for male‐specific amplification and an internal positive control. The primers were designed and tested to be bear‐specific, thereby minimizing the risk of cross‐amplification in other species including humans. The high sensitivity and small amplicon sizes (100, 124, 160 base pairs) facilitate analysis of non‐invasively obtained DNA material. DNA from tissue and blood as well as from 30 non‐invasively collected hair and faeces yielded clear and easily interpretable results. The fragments were detected both by standard gel electrophoresis and automated capillary electrophoresis.
Url:
DOI: 10.1111/1755-0998.12072
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000956
- to stream Istex, to step Curation: 000955
- to stream Istex, to step Checkpoint: 000067
- to stream Main, to step Merge: 000233
- to stream Main, to step Curation: 000232
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non‐invasive samples</title><author><name sortKey="Bidon, Tobias" sort="Bidon, Tobias" uniqKey="Bidon T" first="Tobias" last="Bidon">Tobias Bidon</name></author><author><name sortKey="Frosch, Christiane" sort="Frosch, Christiane" uniqKey="Frosch C" first="Christiane" last="Frosch">Christiane Frosch</name></author><author><name sortKey="Eiken, Hans G" sort="Eiken, Hans G" uniqKey="Eiken H" first="Hans G." last="Eiken">Hans G. Eiken</name></author><author><name sortKey="Kutschera, Verena E" sort="Kutschera, Verena E" uniqKey="Kutschera V" first="Verena E." last="Kutschera">Verena E. Kutschera</name></author><author><name sortKey="Hagen, Snorre B" sort="Hagen, Snorre B" uniqKey="Hagen S" first="Snorre B." last="Hagen">Snorre B. Hagen</name></author><author><name sortKey="Aarnes, Siv G" sort="Aarnes, Siv G" uniqKey="Aarnes S" first="Siv G." last="Aarnes">Siv G. Aarnes</name></author><author><name sortKey="Fain, Steven R" sort="Fain, Steven R" uniqKey="Fain S" first="Steven R." last="Fain">Steven R. Fain</name></author><author><name sortKey="Janke, Axel" sort="Janke, Axel" uniqKey="Janke A" first="Axel" last="Janke">Axel Janke</name></author><author><name sortKey="Hailer, Frank" sort="Hailer, Frank" uniqKey="Hailer F" first="Frank" last="Hailer">Frank Hailer</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:008127C9129A75589E23A050BE6ED181009747DC</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1111/1755-0998.12072</idno><idno type="url">https://api.istex.fr/document/008127C9129A75589E23A050BE6ED181009747DC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000956</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000956</idno><idno type="wicri:Area/Istex/Curation">000955</idno><idno type="wicri:Area/Istex/Checkpoint">000067</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000067</idno><idno type="wicri:doubleKey">1755-098X:2013:Bidon T:a:sensitive:and</idno><idno type="wicri:Area/Main/Merge">000233</idno><idno type="wicri:Area/Main/Curation">000232</idno><idno type="wicri:Area/Main/Exploration">000232</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non‐invasive samples</title><author><name sortKey="Bidon, Tobias" sort="Bidon, Tobias" uniqKey="Bidon T" first="Tobias" last="Bidon">Tobias Bidon</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>LOEWE Biodiversity and Climate Research Centre (BiK‐F), Senckenberg Gesellschaft für Naturforschung, Senckenberganlage 25, 60325, Frankfurt am Main</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country></affiliation></author><author><name sortKey="Frosch, Christiane" sort="Frosch, Christiane" uniqKey="Frosch C" first="Christiane" last="Frosch">Christiane Frosch</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Conservation Genetics Group, Senckenberg Research Institute and Natural History Museum, Clamecystrasse 12, 63571, Gelnhausen</wicri:regionArea><wicri:noRegion>63571, Gelnhausen</wicri:noRegion><wicri:noRegion>Gelnhausen</wicri:noRegion></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Frankfurt Zoological Society, Bernhard‐Grzimek‐Allee 1, 60316, Frankfurt am Main</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Eiken, Hans G" sort="Eiken, Hans G" uniqKey="Eiken H" first="Hans G." last="Eiken">Hans G. Eiken</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Bioforsk Soil and Environment, Svanhovd, Norwegian Institute for Agricultural and Environmental Research, NO‐9925, Svanvik</wicri:regionArea><wicri:noRegion>Svanvik</wicri:noRegion></affiliation></author><author><name sortKey="Kutschera, Verena E" sort="Kutschera, Verena E" uniqKey="Kutschera V" first="Verena E." last="Kutschera">Verena E. Kutschera</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>LOEWE Biodiversity and Climate Research Centre (BiK‐F), Senckenberg Gesellschaft für Naturforschung, Senckenberganlage 25, 60325, Frankfurt am Main</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Hagen, Snorre B" sort="Hagen, Snorre B" uniqKey="Hagen S" first="Snorre B." last="Hagen">Snorre B. Hagen</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Bioforsk Soil and Environment, Svanhovd, Norwegian Institute for Agricultural and Environmental Research, NO‐9925, Svanvik</wicri:regionArea><wicri:noRegion>Svanvik</wicri:noRegion></affiliation></author><author><name sortKey="Aarnes, Siv G" sort="Aarnes, Siv G" uniqKey="Aarnes S" first="Siv G." last="Aarnes">Siv G. Aarnes</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Bioforsk Soil and Environment, Svanhovd, Norwegian Institute for Agricultural and Environmental Research, NO‐9925, Svanvik</wicri:regionArea><wicri:noRegion>Svanvik</wicri:noRegion></affiliation></author><author><name sortKey="Fain, Steven R" sort="Fain, Steven R" uniqKey="Fain S" first="Steven R." last="Fain">Steven R. Fain</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Fish and Wildlife Forensic Laboratory, 1490 East Main Street, OR, 97520, Ashland</wicri:regionArea><wicri:noRegion>Ashland</wicri:noRegion></affiliation></author><author><name sortKey="Janke, Axel" sort="Janke, Axel" uniqKey="Janke A" first="Axel" last="Janke">Axel Janke</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Evolution & Diversity, Goethe University, Institute for Ecology, Siesmayerstrasse 70, 60323, Frankfurt am Main</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Hailer, Frank" sort="Hailer, Frank" uniqKey="Hailer F" first="Frank" last="Hailer">Frank Hailer</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>LOEWE Biodiversity and Climate Research Centre (BiK‐F), Senckenberg Gesellschaft für Naturforschung, Senckenberganlage 25, 60325, Frankfurt am Main</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Ecology Resources</title><title level="j" type="abbrev">Mol Ecol Resour</title><idno type="ISSN">1755-098X</idno><idno type="eISSN">1755-0998</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-05">2013-05</date><biblScope unit="volume">13</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="362">362</biblScope><biblScope unit="page" to="368">368</biblScope></imprint><idno type="ISSN">1755-098X</idno></series><idno type="istex">008127C9129A75589E23A050BE6ED181009747DC</idno><idno type="DOI">10.1111/1755-0998.12072</idno><idno type="ArticleID">MEN12072</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1755-098X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report a new approach for molecular sex identification of extant Ursinae and Tremarctinae bears. Two Y‐specific fragments (SMCY and 318.2) and one X‐specific fragment (ZFX) are amplified in a multiplex PCR, yielding a double test for male‐specific amplification and an internal positive control. The primers were designed and tested to be bear‐specific, thereby minimizing the risk of cross‐amplification in other species including humans. The high sensitivity and small amplicon sizes (100, 124, 160 base pairs) facilitate analysis of non‐invasively obtained DNA material. DNA from tissue and blood as well as from 30 non‐invasively collected hair and faeces yielded clear and easily interpretable results. The fragments were detected both by standard gel electrophoresis and automated capillary electrophoresis.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Norvège</li><li>États-Unis</li></country><region><li>District de Darmstadt</li><li>Hesse (Land)</li></region><settlement><li>Francfort-sur-le-Main</li></settlement></list><tree><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Bidon, Tobias" sort="Bidon, Tobias" uniqKey="Bidon T" first="Tobias" last="Bidon">Tobias Bidon</name></region><name sortKey="Bidon, Tobias" sort="Bidon, Tobias" uniqKey="Bidon T" first="Tobias" last="Bidon">Tobias Bidon</name><name sortKey="Frosch, Christiane" sort="Frosch, Christiane" uniqKey="Frosch C" first="Christiane" last="Frosch">Christiane Frosch</name><name sortKey="Frosch, Christiane" sort="Frosch, Christiane" uniqKey="Frosch C" first="Christiane" last="Frosch">Christiane Frosch</name><name sortKey="Hailer, Frank" sort="Hailer, Frank" uniqKey="Hailer F" first="Frank" last="Hailer">Frank Hailer</name><name sortKey="Hailer, Frank" sort="Hailer, Frank" uniqKey="Hailer F" first="Frank" last="Hailer">Frank Hailer</name><name sortKey="Janke, Axel" sort="Janke, Axel" uniqKey="Janke A" first="Axel" last="Janke">Axel Janke</name><name sortKey="Kutschera, Verena E" sort="Kutschera, Verena E" uniqKey="Kutschera V" first="Verena E." last="Kutschera">Verena E. Kutschera</name></country><country name="Norvège"><noRegion><name sortKey="Eiken, Hans G" sort="Eiken, Hans G" uniqKey="Eiken H" first="Hans G." last="Eiken">Hans G. Eiken</name></noRegion><name sortKey="Aarnes, Siv G" sort="Aarnes, Siv G" uniqKey="Aarnes S" first="Siv G." last="Aarnes">Siv G. Aarnes</name><name sortKey="Hagen, Snorre B" sort="Hagen, Snorre B" uniqKey="Hagen S" first="Snorre B." last="Hagen">Snorre B. Hagen</name></country><country name="États-Unis"><noRegion><name sortKey="Fain, Steven R" sort="Fain, Steven R" uniqKey="Fain S" first="Steven R." last="Fain">Steven R. Fain</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/RenardV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000232 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000232 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= RenardV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:008127C9129A75589E23A050BE6ED181009747DC
|texte= A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non‐invasive samples
}}
| This area was generated with Dilib version V0.6.27. |  |